The University of Nebraska Medical Center has gained international recognition for its expertise in the control and management of highly infectious diseases, with a good deal of public attention given to its work during the 2014 Ebola outbreak in west Africa and now in 2020 with its biocontainment and treatment of more than a dozen cruise ship evacuees who were exposed to the coronavirus. The Nebraska Isolation and Quarantine Manual is a practical guide for local public health officials, emergency management personnel, and health care providers looking to implement evidence-based best practices in the event of an infectious disease outbreak.