This book constitutes the refereed proceedings of the 25th International Conference on Engineering Applications of Neural Networks, EANN 2024, held in Corfu, Greece, during June 27-30, 2024. The 41 full and 2 short papers included in this book were carefully reviewed and selected from 85 submissions. They deal with reinforcement; natural language; biomedical applications; classificaiton; deep learning; convolutional neural networks.